Comparison of Three Commercial Molecular Assays for Detection of Rifampin and Isoniazid Resistance among Mycobacterium tuberculosis Isolates in a High-HIV-Prevalence Setting

K Strydom; F Ismail; M M Z Matabane; O Onwuegbuna; S V Omar; N Ismail

doi:10.1128/JCM.01691-15

Comparison of Three Commercial Molecular Assays for Detection of Rifampin and Isoniazid Resistance among Mycobacterium tuberculosis Isolates in a High-HIV-Prevalence Setting

J Clin Microbiol. 2015 Sep;53(9):3032-4. doi: 10.1128/JCM.01691-15. Epub 2015 Jul 1.

Authors

K Strydom¹, F Ismail², M M Z Matabane², O Onwuegbuna³, S V Omar⁴, N Ismail⁵

Affiliations

¹ Department of Medical Microbiology, University of Pretoria, Pretoria, South Africa National Health Laboratory Services, Tshwane Academic Division, Pretoria, South Africa strydom.kathy@gmail.com.
² Department of Medical Microbiology, University of Pretoria, Pretoria, South Africa National Health Laboratory Services, Tshwane Academic Division, Pretoria, South Africa.
³ National Health Laboratory Services, Tshwane Academic Division, Pretoria, South Africa.
⁴ National Institute for Communicable Diseases: Centre for TB, Johannesburg, South Africa.
⁵ Department of Medical Microbiology, University of Pretoria, Pretoria, South Africa National Institute for Communicable Diseases: Centre for TB, Johannesburg, South Africa.

Abstract

In a head-to-head comparison of the MTBDRplus version 2.0 (Hain Lifescience), the Xpert MTB/RIF (Cepheid), and the Anyplex MTB/NTM (Seegene) assays, we demonstrated equal sensitivity (59/61; 96.7%) and specificity (53/54; 98.1%) for detecting rifampin resistance with further analysis of discordances. The Xpert assay does not detect isoniazid resistance while the Anyplex assay showed high false positivity.

Publication types

Comparative Study
Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Antitubercular Agents / pharmacology*
Drug Resistance, Bacterial*
Genotyping Techniques / methods*
HIV Infections / complications
HIV Infections / epidemiology
Humans
Isoniazid / pharmacology*
Microbial Sensitivity Tests / methods
Mycobacterium tuberculosis / drug effects*
Mycobacterium tuberculosis / isolation & purification
Rifampin / pharmacology*
Sensitivity and Specificity
Tuberculosis / microbiology

Substances

Antitubercular Agents
Isoniazid
Rifampin