Matrix solid phase dispersion method for determination of polycyclic aromatic hydrocarbons in moss

Estefanía Concha-Graña; Soledad Muniategui-Lorenzo; Flavia De Nicola; Jesús R Aboal; Ana Isabel Rey-Asensio; Simonetta Giordano; Ralf Reski; Purificación López-Mahía; Darío Prada-Rodríguez

doi:10.1016/j.chroma.2015.06.014

Matrix solid phase dispersion method for determination of polycyclic aromatic hydrocarbons in moss

J Chromatogr A. 2015 Aug 7:1406:19-26. doi: 10.1016/j.chroma.2015.06.014. Epub 2015 Jun 16.

Authors

Estefanía Concha-Graña¹, Soledad Muniategui-Lorenzo², Flavia De Nicola³, Jesús R Aboal⁴, Ana Isabel Rey-Asensio⁵, Simonetta Giordano⁶, Ralf Reski⁷, Purificación López-Mahía¹, Darío Prada-Rodríguez¹

Affiliations

¹ Grupo Química Analítica Aplicada (QANAP), Instituto Universitario de Medio Ambiente (IUMA), Centro de Investigaciones Científicas Avanzadas (CICA), Departamento de Química Analítica, Facultade de Ciencias, Universidade da Coruña, 15071 A Coruña, Spain.
² Grupo Química Analítica Aplicada (QANAP), Instituto Universitario de Medio Ambiente (IUMA), Centro de Investigaciones Científicas Avanzadas (CICA), Departamento de Química Analítica, Facultade de Ciencias, Universidade da Coruña, 15071 A Coruña, Spain. Electronic address: smuniat@udc.es.
³ Department of Sciences and Technologies, University of Sannio, 82100 Benevento, Italy.
⁴ Department of Cellular Biology and Ecology, Faculty of Biology, University of Santiago de Compostela, 15782 Santiago de Compostela, Spain.
⁵ BIOVIA Consultor Ambiental, Edificio Emprendia, Campus Vida, 15782 Santiago de Compostela, Spain.
⁶ Dipartimento di Biologia, Università di Napoli Federico II, Campus Monte S. Angelo, Via Cintia 4, 80126 Naples, Italy; AMRA S.c.a.r.l., Via Nuova Agnano 11, 80125 Naples, Italy.
⁷ Plant Biotechnology, Faculty of Biology, University of Freiburg, and BIOSS Centre for Biological Signalling Studies, and FRIAS Freiburg Institute for Advances Studies, 79104 Freiburg, Germany.

PMID: 26105781
DOI: 10.1016/j.chroma.2015.06.014

Abstract

In this work a matrix solid-phase dispersion extraction method, followed by programmed temperature vaporization-gas chromatography-tandem mass spectrometry determination is proposed for the analysis of polycyclic aromatic hydrocarbons (PAHs) in moss samples. A devitalized, cultivated Sphagnum palustre L. moss clone obtained from the "Mossclone" EU-FP7 Project was used for the optimization and validation of the proposed method. Good trueness (84-116%), precision (intermediate precision lower than 11%) and sensitivity (quantitation limits lower than 1.7ngg(-1)) were obtained. The proposed method was compared with other procedures applied for this complex matrix, achieving a considerable reduction of sample amount, solvent volume and time consumption. The procedure was successfully tested for the analysis of PAHs in exposed moss clone samples for the monitoring of air pollution. Finally, the method was also tested for its suitability in the analysis of PAHs in other moss species as well as a lichen species.

Keywords: Air-quality; Biomonitoring; Bryophyte; Matrix solid phase dispersion; PTV–GC–MS/MS; Polycyclic aromatic hydrocarbons.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Air Pollutants / analysis*
Bryophyta / chemistry*
Environmental Monitoring / methods*
Gas Chromatography-Mass Spectrometry
Polycyclic Aromatic Hydrocarbons / analysis*
Sensitivity and Specificity
Solid Phase Extraction*

Substances

Air Pollutants
Polycyclic Aromatic Hydrocarbons