This study evaluated the degree of sediment contamination in several South Florida estuaries. During the 2010 National Condition Assessment, Florida Fish and Wildlife Research Institute collected water column, sediment and biotic data from estuaries across the entire state of Florida. Sediments were analyzed for arsenic, cadmium, chromium, lead, mercury, zinc and total polychlorinated biphenyls and were compared relative to empirically derived sediment quality guidelines. As a result of this data collection and assessment effort, it was determined that the degree of contamination with respect to sediment was low for all southern Florida estuaries assessed, except the Miami River which was determined to be considerably contaminated. However only one monitoring location was used to assess the Miami River, and as such should be viewed with caution. A low degree of contamination was determined for Biscayne Bay sediments, possibly indicating a recovery from its previously reported higher contaminant level.