Screening of Propionibacterium spp. for potential probiotic properties

Daniela Campaniello; Antonio Bevilacqua; Milena Sinigaglia; Clelia Altieri

doi:10.1016/j.anaerobe.2015.06.003

Screening of Propionibacterium spp. for potential probiotic properties

Anaerobe. 2015 Aug:34:169-73. doi: 10.1016/j.anaerobe.2015.06.003. Epub 2015 Jun 14.

Authors

Daniela Campaniello¹, Antonio Bevilacqua¹, Milena Sinigaglia¹, Clelia Altieri²

Affiliations

¹ Department of the Science of Agriculture, Food and Environment, University of Foggia, Via Napoli 25, 71122 Foggia, Italy.
² Department of the Science of Agriculture, Food and Environment, University of Foggia, Via Napoli 25, 71122 Foggia, Italy. Electronic address: clelia.altieri@unifg.it.

PMID: 26079323
DOI: 10.1016/j.anaerobe.2015.06.003

Abstract

The main topic of this paper is the evaluation of adhesion of propionibacteria to IPEC-J2 cells and the survival at pH 2.5 and with 0.3% bile salts added, bioactivity towards pathogens and antibiotic resistance of Propionibacterium freudenreichii subsp. shermanii, Propionibacterium jensenii, Propionibacterium acidipropionici and Propionibacterium thoenii. Adhesion to IPEC-J2 cell lines was ca. 25-35% and significantly increased with CaCl2. Moreover, propionibacteria showed a reduction of cell count of ca. 0.5% at pH 2.5 after 3 h, whereas cell count increased after 24 h with bile salts; finally, they significantly inhibited Escherichia coli O157:H7.

Keywords: Adhesion; IPEC-J2 cells; Propionibacteria; Selection criteria.

MeSH terms

Animals
Antibiosis
Bacterial Adhesion*
Bile Acids and Salts / metabolism
Cell Line
Drug Resistance, Bacterial
Epithelial Cells / microbiology*
Hydrogen-Ion Concentration
Microbial Viability / drug effects
Probiotics*
Propionibacterium / drug effects
Propionibacterium / physiology*
Swine

Substances

Bile Acids and Salts