Structure of the neutral capsular polysaccharide of Acinetobacter baumannii NIPH146 that carries the KL37 capsule gene cluster

Nikolay P Arbatsky; Mikhail M Shneider; Johanna J Kenyon; Alexander S Shashkov; Anastasiya V Popova; Konstantin A Miroshnikov; Nikolay V Volozhantsev; Yuriy A Knirel

doi:10.1016/j.carres.2015.05.003

Structure of the neutral capsular polysaccharide of Acinetobacter baumannii NIPH146 that carries the KL37 capsule gene cluster

Carbohydr Res. 2015 Sep 2:413:12-5. doi: 10.1016/j.carres.2015.05.003. Epub 2015 May 21.

Authors

Nikolay P Arbatsky¹, Mikhail M Shneider², Johanna J Kenyon³, Alexander S Shashkov¹, Anastasiya V Popova⁴, Konstantin A Miroshnikov², Nikolay V Volozhantsev⁵, Yuriy A Knirel⁶

Affiliations

¹ N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia.
² M. M. Shemyakin & Y. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
³ School of Molecular Bioscience, The University of Sydney, Sydney, Australia; School of Biomedical Sciences, Queensland University of Technology, Brisbane, Australia.
⁴ Moscow Institute of Physics and Technology, Dolgoprudny, Moscow Region, Russia; State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow Region, Russia.
⁵ State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow Region, Russia.
⁶ N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia. Electronic address: yknirel@gmail.com.

PMID: 26057991
DOI: 10.1016/j.carres.2015.05.003

Abstract

Capsular polysaccharide (CPS) was isolated from Acinetobacter baumannii NIPH146, and the following structure of branched pentasaccharide repeating unit was established by sugar analyses along with 1D and 2D NMR spectroscopy: In comparison to most other known capsular polysaccharides of A. baumannii, the CPS studied is neutral and lacks any specific monosaccharide component. The synthesis, assembly and export of this structure could be attributed to genes in a novel capsule biosynthesis gene cluster, designated KL37, which was found in the NIPH146 genome. The CPS of A. baumannii NIPH146 shares the α-d-Galp-(1→6)-β-d-Glcp-(1→3)-d-GalpNAc-(1→ trisaccharide fragment with the CPS units of several A. baumannii strains, including ATCC 17978 and LUH 5537 that carry the KL3 and KL22 gene clusters, respectively. KL37 contains two genes for glycosyltransferases that are related to two glycosyltransferase genes present in both KL3 and KL22, and the encoded proteins could be tentatively assigned to linkages between sugars in the CPS repeat.

Keywords: Acinetobacter baumannii; Capsular polysaccharide structure; Glycosyltransferase; K locus; KL37 gene cluster.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acinetobacter baumannii / chemistry*
Acinetobacter baumannii / genetics*
Bacterial Capsules / chemistry*
Bacterial Capsules / genetics*
Carbohydrate Sequence
Genetic Loci / genetics
Molecular Sequence Data
Polysaccharides, Bacterial / chemistry*

Substances

Polysaccharides, Bacterial