Structure of the O-specific polysaccharide from a marine bacterium Cellulophaga tyrosinoxydans

Svetlana V Tomshich; Maxim S Kokoulin; Anatoliy I Kalinovsky; Ol'ga I Nedashkovskaya; Nadezhda A Komandrova

doi:10.1016/j.carres.2015.04.024

Structure of the O-specific polysaccharide from a marine bacterium Cellulophaga tyrosinoxydans

Carbohydr Res. 2015 Sep 2:413:1-4. doi: 10.1016/j.carres.2015.04.024. Epub 2015 May 16.

Authors

Svetlana V Tomshich¹, Maxim S Kokoulin², Anatoliy I Kalinovsky², Ol'ga I Nedashkovskaya², Nadezhda A Komandrova²

Affiliations

¹ G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far East Branch of the Russian Academy of Sciences, 690022 Vladivostok, Russian Federation. Electronic address: tomsh@piboc.dvo.ru.
² G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far East Branch of the Russian Academy of Sciences, 690022 Vladivostok, Russian Federation.

PMID: 26048184
DOI: 10.1016/j.carres.2015.04.024

Abstract

The O-polysaccharide was isolated from the lipopolysaccharide of Cellulophaga tyrosinoxydans and studied by chemical analyses along with (1)H and (13)C NMR spectroscopy, including 2D (1)H, (1)H COSY, TOCSY, ROESY, (1)Н, (13)С HSQC, HMBC and H2BC experiments. The following new structure of the O-polysaccharide of C. tyrosinoxydans containing l-fucose (Fuc), N-acetyl-d-glucosamine (GlcNAc), 4-acetamido-4,6-dideoxy-d-glucose (Qui4NAc) and two l-rhamnose residues (Rha) was established:.

Keywords: 4-Acetamido-4,6-dideoxy-d-glucose; Bacterial polysaccharide structure; Cellulophaga tyrosinoxydans; Marine bacterium; O-specific polysaccharide.

MeSH terms

Carbohydrate Sequence
Flavobacteriaceae / chemistry*
Magnetic Resonance Spectroscopy
Molecular Sequence Data
O Antigens / chemistry*

Substances

O Antigens