A miniaturized blotting system for simultaneous detection of different autoantibodies

Methods Mol Biol. 2015:1312:165-73. doi: 10.1007/978-1-4939-2694-7_20.

Abstract

Sera of tumor patients frequently contain autoantibodies to tumor associated antigens. Here we describe a miniaturized immunoblot platform allowing us to screen sera of patients for the presence of autoantibodies to ten autoantigens in parallel.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Autoantibodies / analysis*
  • Autoantibodies / chemistry
  • Autoantibodies / genetics
  • Autoantibodies / immunology
  • Autoantigens / immunology
  • Bacteria / genetics
  • Blotting, Western / methods*
  • Electrophoresis, Polyacrylamide Gel
  • Histidine / chemistry
  • Humans
  • Miniaturization / methods*
  • Time Factors

Substances

  • Autoantibodies
  • Autoantigens
  • Histidine