A novel peptide-based recognition probe for the sensitive detection of CD44 on breast cancer stem cells

Mol Cell Probes. 2015 Dec;29(6):492-499. doi: 10.1016/j.mcp.2015.05.014. Epub 2015 May 30.

Abstract

Metastasis and recurrence of breast cancer remain significant clinical problems. The expression level of CD44 protein is higher in breast cancer-initiating cancer stem cells; therefore, the early detection of CD44 using a sensitive diagnostic probe is important for breast cancer diagnosis and therapeutic purposes. In this study, we fabricated a polyvalent directed peptide polymer (PDPP) that specifically recognized the CD44 biomarker, as confirmed by immunocytochemistry tests and fluorescence-activated cell sorting assessment. Our results indicate that PDPP is useful as a novel tool for the sensitive detection of breast cancer stem cells.

Keywords: Breast cancer stem cell; CD44; PDPP; Peptide.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Breast Neoplasms / diagnosis*
  • Breast Neoplasms / metabolism
  • Cell Line, Tumor
  • Combinatorial Chemistry Techniques / methods
  • Early Detection of Cancer
  • Female
  • Flow Cytometry
  • Humans
  • Hyaluronan Receptors / chemistry
  • Hyaluronan Receptors / metabolism*
  • MCF-7 Cells
  • Mice
  • Molecular Diagnostic Techniques
  • NIH 3T3 Cells
  • Neoplastic Stem Cells / metabolism*
  • Peptides / metabolism*
  • Sensitivity and Specificity
  • Succinimides / chemistry

Substances

  • Hyaluronan Receptors
  • Peptides
  • Succinimides
  • N-succinimidyl 3-(2-pyridyldithio)propionate