New Anti-Inflammatory Cembranes from the Cultured Soft Coral Nephthea columnaris

Ting-Hsi Hsiao; Chun-Sung Sung; Yu-Hsuan Lan; Yi-Chen Wang; Mei-Chin Lu; Zhi-Hong Wen; Yang-Chang Wu; Ping-Jyun Sung

doi:10.3390/md13063443

New Anti-Inflammatory Cembranes from the Cultured Soft Coral Nephthea columnaris

Mar Drugs. 2015 May 29;13(6):3443-53. doi: 10.3390/md13063443.

Authors

Ting-Hsi Hsiao^{1

2}, Chun-Sung Sung^{3

4}, Yu-Hsuan Lan⁵, Yi-Chen Wang^{6

7}, Mei-Chin Lu^{8

9}, Zhi-Hong Wen¹⁰, Yang-Chang Wu^{11

12

13

14}, Ping-Jyun Sung^{15

16

17

18

19}

Affiliations

¹ Graduate Institute of Marine Biology, National Dong Hwa University, Pingtung 944, Taiwan. hsiaoinon@gmail.com.
² National Museum of Marine Biology and Aquarium, Pingtung 944, Taiwan. hsiaoinon@gmail.com.
³ Department of Anesthesiology, Taipei Veterans General Hospital, Taipei 112, Taiwan. sung6119@gmail.com.
⁴ School of Medicine, National Yang-Ming University, Taipei 112, Taiwan. sung6119@gmail.com.
⁵ School of Pharmacy, College of Pharmacy, China Medical University, Taichung 404, Taiwan. lanyh@mail.cmu.edu.tw.
⁶ Department of Marine Biotechnology and Resources, Asia-Pacific Ocean Research Center, National Sun Yat-sen University, Kaohsiung 804, Taiwan. cvyc.wang@gmail.com.
⁷ Division of Cardiology, Department of Internal Medicine, Kaohsiung Armed Forces General Hospital, Kaohsiung 802, Taiwan. cvyc.wang@gmail.com.
⁸ Graduate Institute of Marine Biology, National Dong Hwa University, Pingtung 944, Taiwan. jinx6609@nmmba.gov.tw.
⁹ National Museum of Marine Biology and Aquarium, Pingtung 944, Taiwan. jinx6609@nmmba.gov.tw.
¹⁰ Department of Marine Biotechnology and Resources, Asia-Pacific Ocean Research Center, National Sun Yat-sen University, Kaohsiung 804, Taiwan. wzh@mail.nsysu.edu.tw.
¹¹ School of Pharmacy, College of Pharmacy, China Medical University, Taichung 404, Taiwan. yachwu@mail.cmu.edu.tw.
¹² Chinese Medicine Research and Development Center, China Medical University Hospital, Taichung 404, Taiwan. yachwu@mail.cmu.edu.tw.
¹³ Graduate Institute of Natural Products, Kaohsiung Medical University, Kaohsiung 807, Taiwan. yachwu@mail.cmu.edu.tw.
¹⁴ Center for Molecular Medicine, China Medical University Hospital, Taichung 404, Taiwan. yachwu@mail.cmu.edu.tw.
¹⁵ Graduate Institute of Marine Biology, National Dong Hwa University, Pingtung 944, Taiwan. pjsung@nmmba.gov.tw.
¹⁶ National Museum of Marine Biology and Aquarium, Pingtung 944, Taiwan. pjsung@nmmba.gov.tw.
¹⁷ Department of Marine Biotechnology and Resources, Asia-Pacific Ocean Research Center, National Sun Yat-sen University, Kaohsiung 804, Taiwan. pjsung@nmmba.gov.tw.
¹⁸ Chinese Medicine Research and Development Center, China Medical University Hospital, Taichung 404, Taiwan. pjsung@nmmba.gov.tw.
¹⁹ Graduate Institute of Natural Products, Kaohsiung Medical University, Kaohsiung 807, Taiwan. pjsung@nmmba.gov.tw.

Abstract

Two new cembranes, columnariols A (1) and B (2), were isolated from the cultured soft coral Nephthea columnaris. The structures of cembranes 1 and 2 were elucidated by spectroscopic methods. In the anti-inflammatory effects test, cembranes 1 and 2 were found to significantly inhibit the accumulation of the pro-inflammatory iNOS and COX-2 protein of the lipopolysaccharide (LPS)-stimulated RAW264.7 macrophage cells. Compound 1 exhibited moderate cytotoxicity toward LNCaP cells with an IC50 value of 9.80 μg/mL.

Keywords: COX-2; Nephthea columnaris; antiinflammatory; cembrane; cytotoxicity; iNOS; octocoral.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Anthozoa / chemistry*
Anti-Inflammatory Agents / administration & dosage
Anti-Inflammatory Agents / isolation & purification
Anti-Inflammatory Agents / pharmacology*
Cell Line
Cell Line, Tumor
Cyclooxygenase 2 / metabolism
Diterpenes / administration & dosage
Diterpenes / isolation & purification
Diterpenes / pharmacology*
Humans
Inhibitory Concentration 50
Lipopolysaccharides / pharmacology
Macrophages / drug effects*
Macrophages / metabolism
Mice
Nitric Oxide Synthase Type II / metabolism

Substances

Anti-Inflammatory Agents
Diterpenes
Lipopolysaccharides
Nitric Oxide Synthase Type II
Cyclooxygenase 2