Fostering the action of versatile peroxidase as a highly efficient biocatalyst for the removal of endocrine disrupting compounds

N Biotechnol. 2016 Jan 25;33(1):187-95. doi: 10.1016/j.nbt.2015.05.003. Epub 2015 May 28.

Abstract

Response surface methodology (RSM) was used to optimize the removal of five endocrine disrupting compounds (EDCs) by the enzyme versatile peroxidase (VP): bisphenol A (BPA), triclosan (TCS), estrone (E1), 17β-estradiol (E2) and 17α-ethinylestradiol (EE2). The optimal variables of enzyme activity (90-100 U L(-1)), sodium malonate (29-43 mM) and MnSO4 (0.8-1 mM) led to very high removal rates of the five pollutants (2.5-5.0 mg L(-1) min(-1)). The structural elucidation of transformation products arising from the enzymatic catalysis of the EDCs was investigated by Gas Chromatography coupled to Mass Spectrometry (GC-MS) and Liquid Chromatography Electrospray Time-of-Flight Mass Spectrometry (LC-ESI-TOF-MS). The presence of dimers and trimers, indicative of oxidative coupling, was demonstrated.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biocatalysis*
  • Biodegradation, Environmental
  • Endocrine Disruptors / chemistry
  • Endocrine Disruptors / isolation & purification*
  • Gas Chromatography-Mass Spectrometry
  • Mass Spectrometry
  • Molecular Weight
  • Oxidation-Reduction
  • Peroxidase / metabolism*
  • Regression Analysis
  • Water Pollutants, Chemical / chemistry
  • Water Pollutants, Chemical / isolation & purification

Substances

  • Endocrine Disruptors
  • Water Pollutants, Chemical
  • Peroxidase