The complete chloroplast genome of Armand pine Pinus armandii, an endemic conifer tree species to China

Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Jul;27(4):2635-6. doi: 10.3109/19401736.2015.1041130. Epub 2015 May 29.

Abstract

The complete chloroplast genome (cpDNA) sequence of an endemic conifer species, Armand pine Pinus armandii Franch., is determined in this study. The cpDNA was 117,265 bp in length, containing a pair of 475 bp inverted repeat (IR) regions those distinguished in large and small single copy (LSC and SSC) regions of 64,548 and 51,767 bp in length, respectively. The cpDNA contained 114 genes, including 74 protein-coding genes (74 PCG species), 4 ribosomal RNA genes (four rRNA species) and 36 transfer RNA genes (33 tRNA species). Out of these genes, 12 harbor a single intron and most of the genes occurred in a single copy. The overall AT content of the Armand pine cpDNA was 61.2%, while the corresponding values of the LSC, SSC and IR regions were 62.0%, 60.2% and 62.7%, respectively. A phylogenetic analysis revealed that P. armandii chloroplast genome is closely related to that of the P. koraiensis within the genus Pinus.

Keywords: Chloroplast genome; Illumina sequencing; Pinus armandii; phylogeny.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • AT Rich Sequence / genetics
  • China
  • DNA, Chloroplast / genetics
  • Genome, Chloroplast / genetics*
  • Genome, Mitochondrial / genetics
  • Genome, Plant / genetics*
  • Phylogeny
  • Pinus / classification
  • Pinus / genetics*
  • RNA, Ribosomal / genetics*
  • RNA, Transfer / genetics
  • Sequence Analysis, DNA / methods

Substances

  • DNA, Chloroplast
  • RNA, Ribosomal
  • RNA, Transfer