Production of a new non-specific nuclease from Yersinia enterocolitica subsp. palearctica: optimization of induction conditions using response surface methodology

Xiu-Juan Fang; Zhen-Xing Tang; Zhen-Hua Li; Zhi-Liang Zhang; Lu-E Shi

doi:10.1080/13102818.2014.915612

Production of a new non-specific nuclease from Yersinia enterocolitica subsp. palearctica: optimization of induction conditions using response surface methodology

Biotechnol Biotechnol Equip. 2014 May 4;28(3):559-566. doi: 10.1080/13102818.2014.915612. Epub 2014 Aug 26.

Authors

Xiu-Juan Fang¹, Zhen-Xing Tang², Zhen-Hua Li¹, Zhi-Liang Zhang¹, Lu-E Shi¹

Affiliations

¹ College of Life and Environmental Sciences, Hangzhou Normal University , Hangzhou , Zhejiang , P.R. China.
² College of Life and Environmental Sciences, Hangzhou Normal University , Hangzhou , Zhejiang , P.R. China ; Date Palm Research Center, King Faisal University , Al-hasa , Saudi Arabia ; Department of Food Science, Anqing Vocational & Technical College , Anqing , Anhui , P.R. China.

Abstract

A new non-specific nuclease from Yersinia enterocolitica subsp. palearctica (Y. NSN) was expressed in Escherichia coli (E. coli) BL 21 Star^TM (DE3)plysS. Induction conditions, including isopropyl-β-D-thiogalactoside (IPTG) concentration, cell density (OD600), induction time and induction temperature, were optimized using response surface methodology. Statistical analysis of the results revealed that induction temperature and all the quadratic terms of variables had significant effects on enzyme activity of Y. NSN. The optimal induction conditions were as follows: 1.5 mmol/L IPTG, OD600 of 0.80, induction time of 20.5 h, and induction temperature of 32 °C. Under the optimized conditions, the highest enzyme activity could be obtained.

Keywords: Y. enterocolitica subsp. palearctica; induction conditions; non-specific nuclease; response surface methodology.

Grants and funding

This study was financially supported by the National Natural Science Funds of China [grant number 31100583]; Qianjiang Talent Program of Zhejiang Province [grant number 2012R10060]; Zhejiang Technology Foundation for Selected Overseas Chinese.