Rotavirus

Clin Lab Med. 2015 Jun;35(2):363-91. doi: 10.1016/j.cll.2015.02.012.

Abstract

Group A rotavirus (RVA) is the major cause of acute gastroenteritis (AGE) in young children worldwide. Introduction of two live, attenuated rotavirus vaccines, Rotarix® and RotaTeq®, has dramatically reduced RVA-associated AGE and mortality. High-throughput, sensitive and specific techniques are required to rapidly diagnose and characterize rotavirus strains in stool samples for proper patient treatment and to monitor circulating vaccine and wild-type rotavirus strains. New molecular assays are rapidly developed that are more sensitive and specific than the conventional assays for detection, genotyping and full genome characterization of circulating rotavirus wild-type and vaccine (Rotarix® and RotaTeq®) strains causing AGE.

Keywords: Acute gastroenteritis; Multiplex assays; Next-generation sequencing; RotaTeq®; Rotarix®; Rotavirus; Rotavirus vaccines; qRT-PCR.

Publication types

  • Review

MeSH terms

  • Agglutination Tests
  • Gastroenteritis / diagnosis*
  • Gastroenteritis / epidemiology
  • Gastroenteritis / virology
  • Genotyping Techniques
  • High-Throughput Nucleotide Sequencing
  • Humans
  • Immunoenzyme Techniques
  • Real-Time Polymerase Chain Reaction
  • Reverse Transcriptase Polymerase Chain Reaction
  • Rotavirus / classification
  • Rotavirus / isolation & purification*
  • Rotavirus / physiology
  • Rotavirus Infections / diagnosis*
  • Rotavirus Infections / epidemiology
  • Rotavirus Infections / virology
  • Sequence Analysis, RNA
  • Virus Cultivation / methods