Orthogonally oriented scaffolds with aligned fibers for engineering intestinal smooth muscle

Masae Kobayashi; Nan Ye Lei; Qianqian Wang; Benjamin M Wu; James C Y Dunn

doi:10.1016/j.biomaterials.2015.05.023

Orthogonally oriented scaffolds with aligned fibers for engineering intestinal smooth muscle

Biomaterials. 2015 Aug:61:75-84. doi: 10.1016/j.biomaterials.2015.05.023. Epub 2015 May 15.

Authors

Masae Kobayashi¹, Nan Ye Lei², Qianqian Wang¹, Benjamin M Wu³, James C Y Dunn⁴

Affiliations

¹ Department of Bioengineering, Henry Samueli School of Engineering, University of California, Los Angeles, Los Angeles, CA 90095, USA.
² Department of Bioengineering, Henry Samueli School of Engineering, University of California, Los Angeles, Los Angeles, CA 90095, USA; Department of Surgery, David Geffen School of Medicine at UCLA, University of California, Los Angeles, Los Angeles, CA 90095, USA.
³ Department of Bioengineering, Henry Samueli School of Engineering, University of California, Los Angeles, Los Angeles, CA 90095, USA; Division of Advanced Prosthodontics & Weintraub Center for Reconstructive Biotechnology, University of California, Los Angeles, Los Angeles, CA, USA.
⁴ Department of Bioengineering, Henry Samueli School of Engineering, University of California, Los Angeles, Los Angeles, CA 90095, USA; Department of Surgery, David Geffen School of Medicine at UCLA, University of California, Los Angeles, Los Angeles, CA 90095, USA. Electronic address: jdunn@mednet.ucla.edu.

Abstract

Controlling cellular alignment is critical in engineering intestines with desired structure and function. Although previous studies have examined the directional alignment of cells on the surface (x-y plane) of parallel fibers, quantitative analysis of the cellular alignment inside implanted scaffolds with oriented fibers has not been reported. This study examined the cellular alignment in the x-z and y-z planes of scaffolds made with two layers of orthogonally oriented fibers. The cellular orientation inside implanted scaffolds was evaluated with immunofluorescence. Quantitative analysis of coherency between cell orientation and fiber direction confirmed that cells aligned along the fibers not only on the surface (x-y plane) but also inside the scaffolds (x-z & y-z planes). Our study demonstrated that two layers of orthogonally aligned scaffolds can generate the histological organization of cells similar to that of intestinal circular and longitudinal smooth muscle.

Keywords: Cellular alignment; Electrospinning; Polycaprolactone; Scaffold; Smooth muscle cell; Tissue engineering.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Animals
Anisotropy
Cell Proliferation / physiology
Cells, Cultured
Equipment Design
Equipment Failure Analysis
Intestines / cytology
Intestines / growth & development*
Materials Testing
Mice
Mice, Inbred C57BL
Muscle, Smooth / cytology*
Muscle, Smooth / growth & development*
Myocytes, Smooth Muscle / cytology
Myocytes, Smooth Muscle / physiology*
Nanofibers / chemistry
Nanofibers / ultrastructure*
Tissue Engineering / instrumentation
Tissue Scaffolds*

Abstract

Publication types

MeSH terms

Grants and funding