Fast size-determination of intact bacterial plasmids using nanofluidic channels

K Frykholm; L K Nyberg; E Lagerstedt; C Noble; J Fritzsche; N Karami; T Ambjörnsson; L Sandegren; F Westerlund

doi:10.1039/c5lc00378d

Fast size-determination of intact bacterial plasmids using nanofluidic channels

Lab Chip. 2015 Jul 7;15(13):2739-43. doi: 10.1039/c5lc00378d. Epub 2015 May 21.

Authors

K Frykholm¹, L K Nyberg, E Lagerstedt, C Noble, J Fritzsche, N Karami, T Ambjörnsson, L Sandegren, F Westerlund

Affiliation

¹ Department of Biology and Biological Engineering, Chalmers University of Technology, SE-412 96, Gothenburg, Sweden. fredrik.westerlund@chalmers.se.

PMID: 25997119
DOI: 10.1039/c5lc00378d

Abstract

We demonstrate how nanofluidic channels can be used as a tool to rapidly determine the number and sizes of plasmids in bacterial isolates. Each step can be automated at low cost, opening up opportunities for general use in microbiology labs.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacteria / genetics*
Benzoxazoles / chemistry
DNA, Bacterial / analysis
DNA, Bacterial / chemistry
Nanotechnology / instrumentation
Plasmids / metabolism*
Quinolinium Compounds / chemistry

Substances

Benzoxazoles
DNA, Bacterial
Quinolinium Compounds
1,1'-((4,4,7,7-tetramethyl)-4,7-diazaundecamethylene)bis-4-(3-methyl-2,3-dihydro(benzo-1,3-oxazole)-2-methylidene)quinolinium