Ribonuclease like 5 regulates zebrafish yolk extension by suppressing a p53-dependent DNA damage response pathway

Int J Biochem Cell Biol. 2015 Aug:65:12-9. doi: 10.1016/j.biocel.2015.05.004. Epub 2015 May 14.

Abstract

Ribonuclease like 5 (Rnasel5) is a novel member of the zebrafish ribonuclease A family and its expression is increased during early embryogenesis. However, the in vivo biological function of Rnasel5 remains to be elucidated. Here, we report that knockdown of Rnasel5 by morhpolinos caused shrunken yolk extension as well as increased DNA damage at yolk syncytial layer and external tissue layers via the activation of p53 pathway. In addition, the morphological defects caused by Rnasel5 knockdown can be partially rescued by mRNA injection. Our findings provide the first functional characterization of Rnasel5 in zebrafish development and reveal its critical role in yolk extension by modulation of the p53 pathway.

Keywords: DNA damage; Embryogenesis; P53; Ribonuclease like 5; Yolk extension.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • DNA Damage*
  • Embryo, Nonmammalian
  • Embryonic Development / physiology
  • Gene Knockdown Techniques
  • Ribonuclease, Pancreatic / genetics
  • Ribonuclease, Pancreatic / metabolism*
  • Tumor Suppressor Protein p53 / genetics*
  • Tumor Suppressor Protein p53 / metabolism
  • Yolk Sac / growth & development
  • Yolk Sac / metabolism
  • Yolk Sac / physiology*
  • Zebrafish / physiology*
  • Zebrafish Proteins / genetics*
  • Zebrafish Proteins / metabolism

Substances

  • Tumor Suppressor Protein p53
  • Zebrafish Proteins
  • tp53 protein, zebrafish
  • angiogenin
  • Ribonuclease, Pancreatic