Proteomics methods for discovering viral-host interactions

Anna A Georges; Lori Frappier

doi:10.1016/j.ymeth.2015.05.001

Proteomics methods for discovering viral-host interactions

Methods. 2015 Nov 15:90:21-7. doi: 10.1016/j.ymeth.2015.05.001. Epub 2015 May 7.

Authors

Anna A Georges¹, Lori Frappier²

Affiliations

¹ Department of Molecular Genetics, University of Toronto, 1 Kings College Circle, Toronto M5S 1A8, Canada.
² Department of Molecular Genetics, University of Toronto, 1 Kings College Circle, Toronto M5S 1A8, Canada. Electronic address: lori.frappier@utoronto.ca.

PMID: 25959231
DOI: 10.1016/j.ymeth.2015.05.001

Abstract

The functions of many viral proteins involve direct interactions with specific host proteins. Therefore considerable insight into the functions of a viral protein and its mechanisms of action can come from applying proteomics approaches to viral proteins in order to identify their cellular binding partners. In this chapter we describe proteomics approaches that have proven to be the most useful in identifying host interactions of viral proteins in human cells. Caveats and potential alternatives for each step are also discussed.

Keywords: Affinity purification; Mass spectrometry; Viral proteins.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Host-Pathogen Interactions*
Mass Spectrometry / methods
Proteomics / methods*
Viral Proteins / chemistry*
Viral Proteins / isolation & purification

Substances

Viral Proteins

Grants and funding

Canadian Institutes of Health Research/Canada