Fluorescent sensing of pyrophosphate anion in synovial fluid based on DNA-attached magnetic nanoparticles

Biosens Bioelectron. 2015 Oct 15:72:51-5. doi: 10.1016/j.bios.2015.04.087. Epub 2015 Apr 30.

Abstract

In this work, a new fluorescent method for sensitive detection of pyrophosphate anion (P2O7(4-), PPi) in the synovial fluid was developed using fluorophore labeled single-stranded DNA-attached Fe3O4 NPs. The sensing approach is based on the strong affinity of PPi to Fe3O4 NPs and highly efficient fluorescent quenching ability of Fe3O4 NPs for fluorophore labeled single-stranded DNA. In the presence of PPi, the fluorescence would enhance dramatically due to desorption of fluorophore labeled single-stranded DNA from the surface of Fe3O4 NPs, which allowed the analysis of PPi in a very simple manner. The proposed sensing system allows for the sensitive determination of PPi in the range of 2.0 × 10(-7)-4 × 10(-6)M with a detection limit of 76 nM. Importantly, the protocol exhibits excellent selectivity for the determination of PPi over other phosphate-containing compounds. The method was successfully applied to the determination of PPi in the synovial fluid, which suggests our proposed method has great potential for diagnostic purposes.

Keywords: Fe(3)O(4) nanoparticles; Fluorescent sensing; Fluorophore labeled single-stranded DNA; Pyrophosphate anion; Synovial fluid.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biosensing Techniques / methods
  • DNA, Single-Stranded / chemistry*
  • Diphosphates / analysis*
  • Fluorescent Dyes / chemistry*
  • Humans
  • Immobilized Nucleic Acids / chemistry
  • Limit of Detection
  • Magnetite Nanoparticles / chemistry*
  • Spectrometry, Fluorescence / methods
  • Synovial Fluid / chemistry*

Substances

  • DNA, Single-Stranded
  • Diphosphates
  • Fluorescent Dyes
  • Immobilized Nucleic Acids
  • Magnetite Nanoparticles
  • diphosphoric acid