Determination of free and bound phenolic compounds in soy isoflavone concentrate using a PFP fused core column

Vito Verardo; Ylenia Riciputi; Antonia Garrido-Frenich; Maria Fiorenza Caboni

doi:10.1016/j.foodchem.2015.03.090

Determination of free and bound phenolic compounds in soy isoflavone concentrate using a PFP fused core column

Food Chem. 2015 Oct 15:185:239-44. doi: 10.1016/j.foodchem.2015.03.090. Epub 2015 Mar 28.

Authors

Vito Verardo¹, Ylenia Riciputi², Antonia Garrido-Frenich³, Maria Fiorenza Caboni⁴

Affiliations

¹ Department of Chemistry and Physics (Analytical Chemistry Area), University of Almería, Carretera de Sacramento s/n, E-04120 Almería, Spain; Research Centre for Agricultural and Food Biotechnology (BITAL), Agrifood Campus of International Excellence, ceiA3, Carretera de Sacramento s/n, E-04120 Almería, Spain. Electronic address: vito.verardo@unibo.it.
² Interdepartmental Centre of Agri-food Industrial Research (CIRI Agroalimentare), Alma Mater Studiorum - University of Bologna, Piazza Goidanich 60, I-47521 Cesena (FC), Italy.
³ Department of Chemistry and Physics (Analytical Chemistry Area), University of Almería, Carretera de Sacramento s/n, E-04120 Almería, Spain; Research Centre for Agricultural and Food Biotechnology (BITAL), Agrifood Campus of International Excellence, ceiA3, Carretera de Sacramento s/n, E-04120 Almería, Spain.
⁴ Interdepartmental Centre of Agri-food Industrial Research (CIRI Agroalimentare), Alma Mater Studiorum - University of Bologna, Piazza Goidanich 60, I-47521 Cesena (FC), Italy; Department of Agricultural and Food Sciences, Alma Mater Studiorum - University of Bologna, Piazza Goidanich 60, I-47521 Cesena (FC), Italy.

PMID: 25952864
DOI: 10.1016/j.foodchem.2015.03.090

Abstract

In the last years, the consumption of soy-based foods has increased due to the health benefits related to soy bioactives like phenolic compounds. Thus, in the present study, a new chromatographic method using reverse-phase high performance liquid chromatography coupled to diode array detection (RP-HPLC/DAD) was developed using a fused core pentafluorophenyl (PFP) column. The established method allowed the determination of twenty-one free phenolic compounds and eleven bound phenolics in a soy isoflavone concentrate. The method was validated in terms of precision and recovery. Intra and inter-day precision were less than 5% (% RSD) and the recovery was between 97.4% and 103.6%. Limits of quantification (LOQs) ranged between 0.093 and 0.443 μg/mL. Because of that, PFP stationary phase can be easily applied for routine determination of phenolic compounds in soy based foods.

Keywords: Fused core column; Isoflavones; PFP; Phenolic acids; Soybean.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chromatography, High Pressure Liquid / methods*
Glycine max / chemistry*
Isoflavones / analysis*
Limit of Detection
Phenols / analysis*
Spectrometry, Mass, Electrospray Ionization

Substances

Isoflavones
Phenols