Spiroboronate Si-rhodamine as a near-infrared probe for imaging lysosomes based on the reversible ring-opening process

Chem Commun (Camb). 2015 Jun 14;51(47):9608-11. doi: 10.1039/c5cc02496j.

Abstract

A cyclic boronate structure was incorporated into Si-rhodamine to design a pH-activatable near-infrared (NIR) probe based on the reversible ring-opening process triggered by H(+). The probe showed general suitability of specific labelling of acidic lysosomes and tracking their pH changes in living cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Fluorescent Dyes / chemistry*
  • Fluorescent Dyes / metabolism
  • Hep G2 Cells
  • Humans
  • Hydrogen-Ion Concentration
  • Image Processing, Computer-Assisted / methods*
  • Lysosomes / metabolism*
  • Molecular Imaging
  • PC12 Cells
  • Rats
  • Rhodamines / chemistry*
  • Rhodamines / metabolism
  • Spectrometry, Fluorescence
  • Spectroscopy, Near-Infrared
  • Spironolactone / chemistry*
  • Spironolactone / metabolism

Substances

  • Fluorescent Dyes
  • Rhodamines
  • Spironolactone