Multivalent aptamer-RNA based fluorescent probes for carrier-free detection of cellular microRNA-34a in mucin1-expressing cancer cells

Chem Commun (Camb). 2015 May 28;51(43):9038-41. doi: 10.1039/c5cc02052b.

Abstract

In this study, multivalent carrier-free aptamer-RNA based fluorescent probes (CF-probes) were designed as a simpler, more reliable, timesaving strategy for cellular miRNA detection. CF-probes spontaneously delivered into cells without the need for additional carriers and visualized target microRNA-34a specifically.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aptamers, Nucleotide / chemistry*
  • Cell Line, Tumor
  • Electrophoresis, Polyacrylamide Gel
  • Flow Cytometry
  • Fluorescent Dyes / chemistry
  • Humans
  • MCF-7 Cells
  • MicroRNAs / analysis*
  • MicroRNAs / blood
  • MicroRNAs / urine
  • Microscopy, Confocal
  • Mucin-1 / metabolism*
  • Oligonucleotides / chemistry
  • Spectrometry, Fluorescence

Substances

  • Aptamers, Nucleotide
  • Fluorescent Dyes
  • MicroRNAs
  • Mucin-1
  • Oligonucleotides
  • locked nucleic acid