Immunoblotting conditions for small peptides from streptococci

Abstract

Streptococci secrete small peptides with important biological functions. These peptides are not amenable to standard immunoblotting, and are often detected indirectly using activity assays, or by alternative approaches that may be expensive and laborious. Here we describe an immunoblotting method that enables reproducible detection of these small streptococcal peptides.

Keywords: Bacteriocins; Competence stimulating peptides; Immunoblotting; Streptococcal small peptides.