Abstract
The results of the construction of the first Russian ELISA system for the quantitative determination of D-antigen of 1-3 poliovirus types in the preparations of inactivated poliovirus vaccines are presented. For the first time, this kind of system is based on the use of specific antibodies of class Y (IgY) from egg yolks of immunized hens. It was shown that this ELISA system is specific, sufficiently sensitive, and can be used for quantitative determination of D-antigen of 1-3 poliovirus types in inactivated poliovirus vaccines.
MeSH terms
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Animals
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Antibodies, Viral / analysis*
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Antibodies, Viral / biosynthesis
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Antibodies, Viral / isolation & purification
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Antigens, Viral / immunology*
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Chickens / immunology
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Egg Yolk / chemistry
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Egg Yolk / immunology
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Enzyme-Linked Immunosorbent Assay / methods*
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Enzyme-Linked Immunosorbent Assay / standards
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Female
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Humans
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Immunization
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Immunoglobulins / analysis*
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Immunoglobulins / biosynthesis
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Immunoglobulins / isolation & purification
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Poliomyelitis / diagnosis*
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Poliomyelitis / immunology
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Poliomyelitis / prevention & control*
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Poliomyelitis / virology
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Poliovirus / immunology
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Poliovirus Vaccine, Inactivated / administration & dosage
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Sensitivity and Specificity
Substances
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Antibodies, Viral
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Antigens, Viral
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IgY
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Immunoglobulins
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Poliovirus Vaccine, Inactivated