[An ELISA system based on the specific class Y (IgY) antibodies from egg yolks for the quantitative determination of D-antigen in inactivated poliovirus vaccines]

Vopr Virusol. 2014;59(6):39-42.
[Article in Russian]

Abstract

The results of the construction of the first Russian ELISA system for the quantitative determination of D-antigen of 1-3 poliovirus types in the preparations of inactivated poliovirus vaccines are presented. For the first time, this kind of system is based on the use of specific antibodies of class Y (IgY) from egg yolks of immunized hens. It was shown that this ELISA system is specific, sufficiently sensitive, and can be used for quantitative determination of D-antigen of 1-3 poliovirus types in inactivated poliovirus vaccines.

Publication types

  • English Abstract

MeSH terms

  • Animals
  • Antibodies, Viral / analysis*
  • Antibodies, Viral / biosynthesis
  • Antibodies, Viral / isolation & purification
  • Antigens, Viral / immunology*
  • Chickens / immunology
  • Egg Yolk / chemistry
  • Egg Yolk / immunology
  • Enzyme-Linked Immunosorbent Assay / methods*
  • Enzyme-Linked Immunosorbent Assay / standards
  • Female
  • Humans
  • Immunization
  • Immunoglobulins / analysis*
  • Immunoglobulins / biosynthesis
  • Immunoglobulins / isolation & purification
  • Poliomyelitis / diagnosis*
  • Poliomyelitis / immunology
  • Poliomyelitis / prevention & control*
  • Poliomyelitis / virology
  • Poliovirus / immunology
  • Poliovirus Vaccine, Inactivated / administration & dosage
  • Sensitivity and Specificity

Substances

  • Antibodies, Viral
  • Antigens, Viral
  • IgY
  • Immunoglobulins
  • Poliovirus Vaccine, Inactivated