Targeting intracellular bacteria with an extended cationic amphiphilic polyproline helix

Org Biomol Chem. 2015 Jun 7;13(21):5930-6. doi: 10.1039/c5ob00227c.

Abstract

An extended cationic and amphiphilic polyproline helix (CAPH) is described with a dual mode of action: effective cell penetration of human macrophages, and potent antimicrobial activity in vitro against both Gram-positive and negative pathogens, including Acinetobacter baumannii, Escherichia coli O157 and Bacillus anthracis. This dual action was successfully combined to clear pathogenic bacteria (Brucella and Salmonella) residing within macrophages.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Anti-Bacterial Agents / chemistry*
  • Anti-Bacterial Agents / pharmacokinetics
  • Anti-Bacterial Agents / pharmacology*
  • Antimicrobial Cationic Peptides / chemistry*
  • Antimicrobial Cationic Peptides / pharmacokinetics
  • Antimicrobial Cationic Peptides / pharmacology*
  • Bacteria / drug effects
  • Bacterial Infections / drug therapy
  • Cell Line
  • Humans
  • Macrophages / microbiology*
  • Microbial Sensitivity Tests
  • Models, Molecular
  • Peptides / chemistry*
  • Peptides / pharmacokinetics
  • Peptides / pharmacology*

Substances

  • Anti-Bacterial Agents
  • Antimicrobial Cationic Peptides
  • Peptides
  • polyproline