Multimodal noncontact photoacoustic and optical coherence tomography imaging using wavelength-division multiplexing

J Biomed Opt. 2015 Apr;20(4):46013. doi: 10.1117/1.JBO.20.4.046013.

Abstract

We present multimodal noncontact photoacoustic (PA) and optical coherence tomography (OCT) imaging. PA signals are acquired remotely on the surface of a specimen with a Mach-Zehnder interferometer. The interferometer is realized in a fiber-optic network using a fiber laser at 1550 nm as the source. In the same fiber-optic network, a spectral-domain OCT system is implemented. The OCT system utilizes a supercontinuum light source at 1310 nm and a spectrometer with an InGaAs line array detector. Light from the fiber laser and the OCT source is multiplexed into one fiber using a wavelength-division multiplexer; the same objective is used for both imaging modalities. Reflected light is spectrally demultiplexed and guided to the respective imaging systems. We demonstrate two-dimensional and three-dimensional imaging on a tissue-mimicking sample and a chicken skin phantom. The same fiber network and same optical components are used for PA and OCT imaging, and the obtained images are intrinsically coregistered.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Chickens
  • Fiber Optic Technology / instrumentation*
  • Models, Biological
  • Phantoms, Imaging
  • Photoacoustic Techniques / instrumentation
  • Photoacoustic Techniques / methods*
  • Skin / chemistry
  • Tomography, Optical Coherence / instrumentation
  • Tomography, Optical Coherence / methods*