New flavone glycoside, genkwanin 4'-O-β-glucopyranosyl-(1 --> 2)-O-α-rhamnopyranoside was isolated from the fronds of new chemotype of Asplenium normale D. Don, together with two known C-glycosylflavones, vicenin-2 and lucenin-2. The chemical structure of the isolated glycoside was established by UV, LC-MS, characterization of acid hydrolysates, and 1H and 13C NMR spectroscopy.