Structure of the K6 capsular polysaccharide from Acinetobacter baumannii isolate RBH4

Carbohydr Res. 2015 May 29:409:30-5. doi: 10.1016/j.carres.2015.03.016. Epub 2015 Apr 6.

Abstract

The structure of the capsular polysaccharide (CPS) from an Acinetobacter baumannii global clone 2 (GC2) clinical isolate RBH4 that carries the KL6 gene cluster was elucidated by means of chemical and spectroscopical methods. The repeating unit of K6 CPS is linear and contains N-acetyl-D-galactosamine (D-GalpNAc), two D-galactose (D-Galp) residues and 5,7-di-N-acetylpseudaminic acid (Pse5Ac7Ac). The synthesis of these sugars could be attributed to genes in the KL6 capsule biosynthesis gene cluster, and the formation of the linkages between the sugars were assigned to glycosyltransferases or the Wzy polymerase encoded in KL6.

Keywords: Capsular polysaccharide; K locus; KL6 gene cluster; NMR spectroscopy; Pseudaminic acid.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acinetobacter baumannii / chemistry*
  • Acinetobacter baumannii / genetics
  • Magnetic Resonance Spectroscopy
  • Multigene Family / genetics
  • Sugar Acids / chemistry

Substances

  • 5,7-diacetamido-3,5,7,9-tetradeoxynonulosonic acid
  • Sugar Acids