Salinity induced oxidative stress enhanced biofuel production potential of microalgae Scenedesmus sp. CCNM 1077

Imran Pancha; Kaumeel Chokshi; Rahulkumar Maurya; Khanjan Trivedi; Shailesh Kumar Patidar; Arup Ghosh; Sandhya Mishra

doi:10.1016/j.biortech.2015.04.017

Salinity induced oxidative stress enhanced biofuel production potential of microalgae Scenedesmus sp. CCNM 1077

Bioresour Technol. 2015:189:341-348. doi: 10.1016/j.biortech.2015.04.017. Epub 2015 Apr 16.

Authors

Imran Pancha¹, Kaumeel Chokshi¹, Rahulkumar Maurya¹, Khanjan Trivedi², Shailesh Kumar Patidar³, Arup Ghosh², Sandhya Mishra⁴

Affiliations

¹ Discipline of Salt & Marine Chemicals, CSIR - Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India; Academy of Scientific & Innovative Research (AcSIR), CSIR - Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India.
² Academy of Scientific & Innovative Research (AcSIR), CSIR - Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India; Discipline of Wasteland Research, CSIR- Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India.
³ Discipline of Salt & Marine Chemicals, CSIR - Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India.
⁴ Discipline of Salt & Marine Chemicals, CSIR - Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India; Academy of Scientific & Innovative Research (AcSIR), CSIR - Central Salt and Marine Chemicals Research Institute, Bhavnagar 364002, India. Electronic address: smishra@csmcri.org.

PMID: 25911594
DOI: 10.1016/j.biortech.2015.04.017

Abstract

Microalgal biomass is considered as potential feedstock for biofuel production. Enhancement of biomass, lipid and carbohydrate contents in microalgae is important for the commercialization of microalgal biofuels. In the present study, salinity stress induced physiological and biochemical changes in microalgae Scenedesmus sp. CCNM 1077 were studied. During single stage cultivation, 33.13% lipid and 35.91% carbohydrate content was found in 400 mM NaCl grown culture. During two stage cultivation, salinity stress of 400 mM for 3 days resulted in 24.77% lipid (containing 74.87% neutral lipid) along with higher biomass compared to single stage, making it an efficient strategy to enhance biofuel production potential of Scenedesmus sp. CCNM 1077. Apart from biochemical content, stress biomarkers like hydrogen peroxide, lipid peroxidation, ascorbate peroxidase, proline and mineral contents were also studied to understand the role of reactive oxygen species (ROS) mediated lipid accumulation in microalgae Scenedesmus sp. CCNM 1077.

Keywords: Carbohydrate; Lipid; Microalgae; Oxidative stress; Salinity.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Ascorbate Peroxidases / metabolism
Biofuels / microbiology*
Biomarkers / metabolism
Biomass
Biotechnology / methods*
Hydrogen Peroxide / metabolism
Lipids / analysis
Malondialdehyde / metabolism
Microalgae / drug effects
Microalgae / metabolism*
Minerals / metabolism
Oxidative Stress* / drug effects
Pigments, Biological / metabolism
Proline / metabolism
Salinity*
Scenedesmus / drug effects
Scenedesmus / metabolism*
Sodium Chloride / pharmacology

Substances

Biofuels
Biomarkers
Lipids
Minerals
Pigments, Biological
Sodium Chloride
Malondialdehyde
Proline
Hydrogen Peroxide
Ascorbate Peroxidases