Genetic Incorporation of ε-N-2-Hydroxyisobutyryl-lysine into Recombinant Histones

ACS Chem Biol. 2015 Jul 17;10(7):1599-603. doi: 10.1021/cb501055h. Epub 2015 Apr 29.

Abstract

Here, we report the evolution of an orthogonal amber suppressor pyrrolysyl-tRNA synthetase (PylRS)/tRNACUA(Pyl) pair that genetically encodes the post-translationally modified amino acid, ε-N-2-hydroxyisobutyryl-lysine (HibK), in bacteria and mammalian cells. HibK is a new type of histone mark that is widely distributed in histone proteins. The ability to site-specifically incorporate HibK into proteins provides a useful tool to probe the biological function of this newly identified post-translational modification.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Amino Acyl-tRNA Synthetases / genetics
  • Amino Acyl-tRNA Synthetases / metabolism
  • Animals
  • Escherichia coli / genetics
  • Escherichia coli / metabolism
  • Gene Expression
  • HEK293 Cells
  • Histones / genetics*
  • Histones / metabolism
  • Humans
  • Lysine / analogs & derivatives*
  • Lysine / genetics*
  • Models, Molecular
  • Protein Processing, Post-Translational
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism

Substances

  • Histones
  • Recombinant Proteins
  • Amino Acyl-tRNA Synthetases
  • Lysine