Characterization and real-time imaging of the FTLD-related protein aggregation induced by amyloidogenic peptides

Chem Commun (Camb). 2015 May 21;51(41):8652-5. doi: 10.1039/c5cc00513b.

Abstract

We identify a new amyloidogenic peptide from the glutamine/asparagine-rich region of the FTLD-related protein (TDP-43), which can seed both the full-length and N-terminus-truncated TDP-43. Through the microinjection and real-time fluorescence imaging, we also found that this novel peptide could trigger cell apoptosis and initiate TDP-43 aggregation in the cytosol.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amyloidogenic Proteins / chemistry*
  • Amyloidogenic Proteins / pharmacology*
  • Animals
  • Apoptosis / drug effects
  • Cell Line, Transformed
  • Cytosol / metabolism
  • DNA-Binding Proteins / chemistry*
  • Fluorescence
  • Fluorescent Dyes / chemistry
  • Frontotemporal Lobar Degeneration
  • Humans
  • Molecular Structure
  • Protein Aggregates / drug effects*
  • Protein Aggregation, Pathological / chemically induced*
  • Rats
  • Spectrometry, Fluorescence
  • Time Factors

Substances

  • Amyloidogenic Proteins
  • DNA-Binding Proteins
  • Fluorescent Dyes
  • Protein Aggregates
  • TARDBP protein, human