Muscle extracellular matrix scaffold is a multipotent environment

Paola Aulino; Alessandra Costa; Ernesto Chiaravalloti; Barbara Perniconi; Sergio Adamo; Dario Coletti; Massimo Marrelli; Marco Tatullo; Laura Teodori

doi:10.7150/ijms.10761

Muscle extracellular matrix scaffold is a multipotent environment

Int J Med Sci. 2015 Apr 6;12(4):336-40. doi: 10.7150/ijms.10761. eCollection 2015.

Authors

Paola Aulino¹, Alessandra Costa², Ernesto Chiaravalloti³, Barbara Perniconi⁴, Sergio Adamo⁵, Dario Coletti⁴, Massimo Marrelli³, Marco Tatullo⁶, Laura Teodori⁷

Affiliations

¹ 1. Section of Histology and Medical Embryology, Department of Anatomical, Histological, Forensic and Orthopaedic Sciences, Sapienza University of Rome, Rome, Italy ; 2. Calabrodental clinic, Biomedical Section, Maxillofacial Surgery Unit, Crotone, Italy.
² 1. Section of Histology and Medical Embryology, Department of Anatomical, Histological, Forensic and Orthopaedic Sciences, Sapienza University of Rome, Rome, Italy ; 3. Fondazione San Raffaele, Ceglie Messapica, Italy ; 4. Department of Surgery, McGowan Institute, University of Pittsburgh Medical Center, Pittsburgh, PA, USA.
³ 2. Calabrodental clinic, Biomedical Section, Maxillofacial Surgery Unit, Crotone, Italy.
⁴ 2. Calabrodental clinic, Biomedical Section, Maxillofacial Surgery Unit, Crotone, Italy ; 5. UMR 8256 CNRS Biology of Adaptation and Aging, University Pierre et Marie Curie Paris 06, Paris, France.
⁵ 1. Section of Histology and Medical Embryology, Department of Anatomical, Histological, Forensic and Orthopaedic Sciences, Sapienza University of Rome, Rome, Italy.
⁶ 2. Calabrodental clinic, Biomedical Section, Maxillofacial Surgery Unit, Crotone, Italy ; 6. Tecnologica Research Institute, Biomedical Section, Crotone, Italy.
⁷ 3. Fondazione San Raffaele, Ceglie Messapica, Italy ; 7. UTAPRAD-DIM, ENEA Frascati, Rome, Italy.

Abstract

The multipotency of scaffolds is a new concept. Skeletal muscle acellular scaffolds (MAS) implanted at the interface of Tibialis Anterior/tibial bone and masseter muscle/mandible bone in a murine model were colonized by muscle cells near the host muscle and by bone-cartilaginous tissues near the host bone, thus highlighting the importance of the environment in directing cell homing and differentiation. These results unveil the multipotency of MAS and point to the potential of this new technique as a valuable tool in musculo-skeletal tissue regeneration.

Keywords: ECM scaffold; bone; cartilage; skeletal muscle; tissue engineering.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Differentiation
Cell Movement
Extracellular Matrix / chemistry*
Female
Male
Mice
Mice, Inbred Strains
Models, Animal
Multipotent Stem Cells / cytology
Muscle, Skeletal / cytology
Muscle, Skeletal / physiology*
Myoblasts, Skeletal / cytology
Regeneration
Stem Cell Niche
Tissue Engineering / methods
Tissue Scaffolds / chemistry*

Abstract

Publication types

MeSH terms

Grants and funding