Abstract
Phytochemical investigation of the twigs of Chaenomeles sinensis led to the isolation and identification of six new lignan glycosides, chaenomiside A-F (1-6), along with five known ones (7-11). Their chemical structures were determined by spectroscopic methods, including NMR, MS, ECD, and GC/MS analyses. All the isolated compounds (1-11) were tested for their inhibitory effects on nitric oxide (NO) production in lipopolysaccharide-activated murine microglial cells and the secretion of nerve growth factor (NGF) in a C6 rat glioma cell line. Compound 6 significantly reduced NO levels in the murine microglia BV2 cells with an IC50 value of 21.3 μM, and compounds 1, 3, and 6 were potent stimulants of NGF release with stimulation levels of 151.74 ± 6.77%, 144.31 ± 7.49%, and 167.61 ± 18.5%, respectively.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Anti-Inflammatory Agents / chemistry
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Anti-Inflammatory Agents / isolation & purification*
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Anti-Inflammatory Agents / pharmacology
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Glycosides / chemistry
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Glycosides / isolation & purification*
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Glycosides / pharmacology*
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Inhibitory Concentration 50
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Lignans / chemistry
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Lignans / isolation & purification*
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Lignans / pharmacology*
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Lipopolysaccharides / chemistry
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Lipopolysaccharides / isolation & purification*
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Lipopolysaccharides / pharmacology*
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Macrophages / drug effects
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Mice
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Microglia / drug effects
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Molecular Structure
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Nerve Growth Factor / metabolism
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Nitric Oxide / analysis
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Nitric Oxide / biosynthesis
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Plant Stems / chemistry
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Rats
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Republic of Korea
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Rosaceae / chemistry*
Substances
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Anti-Inflammatory Agents
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Glycosides
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Lignans
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Lipopolysaccharides
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Nitric Oxide
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Nerve Growth Factor