EGFR/ERBB receptors differentially modulate sebaceous lipogenesis

Maik Dahlhoff; Emanuela Camera; Matteo Ludovici; Mauro Picardo; Udo Müller; Heinrich Leonhardt; Christos C Zouboulis; Marlon R Schneider

doi:10.1016/j.febslet.2015.04.003

EGFR/ERBB receptors differentially modulate sebaceous lipogenesis

FEBS Lett. 2015 May 22;589(12):1376-82. doi: 10.1016/j.febslet.2015.04.003. Epub 2015 Apr 15.

Authors

Maik Dahlhoff¹, Emanuela Camera², Matteo Ludovici², Mauro Picardo², Udo Müller³, Heinrich Leonhardt³, Christos C Zouboulis⁴, Marlon R Schneider⁵

Affiliations

¹ Institute of Molecular Animal Breeding and Biotechnology, Gene Center, LMU Munich, Munich, Germany.
² Laboratory of Cutaneous Physiopathology and Integrated Center of Metabolomics Research, San Gallicano Dermatologic Institute, IRCCS, Rome, Italy.
³ Human Biology and BioImaging, Department of Biology II, LMU Munich, Germany.
⁴ Departments of Dermatology, Venereology, Allergology and Immunology, Dessau Medical Center, Dessau, Germany.
⁵ Institute of Molecular Animal Breeding and Biotechnology, Gene Center, LMU Munich, Munich, Germany. Electronic address: marlon.schneider@lmu.de.

PMID: 25889637
DOI: 10.1016/j.febslet.2015.04.003

Abstract

The roles of the epidermal growth factor receptor (EGFR) in sebaceous glands remain poorly explored. We show that human sebocytes express EGFR and lower levels of ERBB2 and ERBB3, all receptors being downregulated after the induction of lipid synthesis. Nile red staining showed that siRNA-mediated downregulation of EGFR or ERBB3 increases lipid accumulation, whereas ERBB2 downregulation has no effect. Spectrometry confirmed induction of triglycerides after EGFR or ERBB3 downregulation and revealed induction of cholesteryl esters after downregulation of EGFR, ERBB2 or ERBB3. Thus, EGFR/ERBB receptors differentially modulate sebaceous lipogenesis, a key feature of sebaceous gland physiology and of several skin diseases.

Keywords: ERBB; Epidermal growth factor receptor; Sebaceous gland; Sebaceous lipogenesis.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Biomarkers / metabolism
Cell Line
Cholesterol Esters / metabolism
Down-Regulation
ErbB Receptors / antagonists & inhibitors
ErbB Receptors / genetics
ErbB Receptors / metabolism*
Fatty Acids, Nonesterified / metabolism
Gene Expression Regulation, Enzymologic*
Humans
Ligands
Linoleic Acid / metabolism
Lipogenesis*
RNA Interference
RNA, Small Interfering
Receptor, ErbB-2 / antagonists & inhibitors
Receptor, ErbB-2 / genetics
Receptor, ErbB-2 / metabolism*
Receptor, ErbB-3 / antagonists & inhibitors
Receptor, ErbB-3 / genetics
Receptor, ErbB-3 / metabolism*
Sebaceous Glands / enzymology
Sebaceous Glands / metabolism*
Triglycerides / metabolism
Up-Regulation

Substances

Biomarkers
Cholesterol Esters
Fatty Acids, Nonesterified
Ligands
RNA, Small Interfering
Triglycerides
Linoleic Acid
EGFR protein, human
ERBB2 protein, human
ERBB3 protein, human
ErbB Receptors
Receptor, ErbB-2
Receptor, ErbB-3