Abstract
Dysregulation of microRNAs (miRs) is involved in carcinogenesis. Deregulation of miR-211 has recently been observed in many tumors, but its function in hepatocellular carcinoma (HCC) is still unknown. Here we found that miR-211 was decreased in HCC cancer tissues compared with adjacent normal tissues. We also found that overexpression of miR-211 repressed proliferation and invasion in HepG2 and SMMC7721 cells. Luciferase reporter assays and western blot indicated that special AT-rich sequence-binding protein-2 (SATB2), is a direct target of miR-211. The expression of SATB2 was upregulated in HCC cancer tissues and cell lines and miR-211 levels inversely correlated with SATB2 levels in HCC. Importantly, SATB2 rescued the miR-211-mediated inhibition of cell invasion and proliferation. Finally, reintroduction of miR-211 repressed tumor formation of HCC in xenograft mice. This study provides insights into molecular mechanisms that miR-211 contributed to HCC.
Keywords:
SATB2; hepatocellular carcinoma; miR-211; microRNAs.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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3' Untranslated Regions / genetics
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Adenocarcinoma / pathology
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Adult
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Aged
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Animals
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Carcinoma, Hepatocellular / genetics*
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Carcinoma, Hepatocellular / metabolism
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Carcinoma, Hepatocellular / pathology
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Carcinoma, Hepatocellular / secondary
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Cell Line, Tumor
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Down-Regulation
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Female
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Gene Expression Regulation, Neoplastic*
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Hep G2 Cells
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Heterografts
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Humans
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Liver Neoplasms / genetics*
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Liver Neoplasms / metabolism
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Liver Neoplasms / pathology
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Lymphatic Metastasis
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Male
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Matrix Attachment Region Binding Proteins / biosynthesis*
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Matrix Attachment Region Binding Proteins / genetics
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Mice
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MicroRNAs / genetics*
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Middle Aged
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Neoplasm Invasiveness
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Neoplasm Proteins / biosynthesis*
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Neoplasm Proteins / genetics
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RNA, Messenger / biosynthesis
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RNA, Messenger / genetics
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RNA, Neoplasm / biosynthesis
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RNA, Neoplasm / genetics*
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Transcription Factors / biosynthesis*
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Transcription Factors / genetics
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Transfection
Substances
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3' Untranslated Regions
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MIRN211 microRNA, human
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Matrix Attachment Region Binding Proteins
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MicroRNAs
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Neoplasm Proteins
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RNA, Messenger
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RNA, Neoplasm
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SATB2 protein, human
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Transcription Factors