Molecular cloning and functional characterization of cyclophilin A in yellow catfish (Pelteobagrus fulvidraco)

Fish Shellfish Immunol. 2015 Aug;45(2):422-30. doi: 10.1016/j.fsi.2015.04.002. Epub 2015 Apr 13.

Abstract

Cyclophilin A (CypA) is a ubiquitously expressed protein which involves in diverse pathological conditions including infection and inflammation. In this report, a CypA gene (designated as YC-CypA) was cloned from yellow catfish (Pelteobagrus fulvidraco) which is an important cultured fish species in Asian countries. The open reading frame (ORF) of YC-CypA encoded a polypeptide of 164 amino acids with calculated molecular weight of 17.70 kDa. The deduced amino acid sequences of the YC-CypA shared highly conserved structures with CypAs from the other species, indicating that YC-CypA should be a new member of the CypA family. Full-length YC-CypA protein was expressed in Escherichia coli and specific polyclonal antibody against YC-CypA was generated. The YC-CypA protein showed chemotactic activity by transwell migration assay. The mRNA and protein of YC-CypA could be detected in all examined tissues with relatively higher mRNA level in spleen and higher protein level in head kidney, respectively. The temporal expression patterns of YC-CypA, IL-1β and TNF-α mRNAs were analyzed in the liver, spleen and head kidney post of Edwardsiella ictaluri infection. By immunohistochemistry assay, slight enhancement of YC-CypA protein was observed in the liver, spleen, body kidney and head kidney of yellow catfish infected with E. ictaluri. In conclusion, YC-CypA of yellow catfish showed chemotactic activity in vitro and might have been involved in cytokines secretion in yellow catfish during the infection of E. ictaluri.

Keywords: Chemotactic activity; Cyclophilin A; Edwardsiella ictaluri; Expression; Pelteobagrus fulvidraco.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Catfishes* / genetics
  • Catfishes* / immunology
  • Catfishes* / metabolism
  • Cell Movement
  • Cloning, Molecular
  • Cyclophilin A* / genetics
  • Cyclophilin A* / immunology
  • Cyclophilin A* / metabolism
  • Edwardsiella ictaluri
  • Enterobacteriaceae Infections / immunology
  • Enterobacteriaceae Infections / metabolism
  • Fish Diseases / immunology
  • Fish Diseases / metabolism
  • Fish Proteins* / genetics
  • Fish Proteins* / immunology
  • Fish Proteins* / metabolism
  • Head Kidney / metabolism
  • Interleukin-1beta / genetics
  • Kidney / metabolism
  • Leukocytes / immunology
  • Liver / metabolism
  • Molecular Sequence Data
  • Phylogeny
  • RNA, Messenger / metabolism
  • Spleen / metabolism
  • Tumor Necrosis Factor-alpha / genetics

Substances

  • Fish Proteins
  • Interleukin-1beta
  • RNA, Messenger
  • Tumor Necrosis Factor-alpha
  • Cyclophilin A

Associated data

  • GENBANK/KP119459