Cloning and sequence analysis of sucrose phosphate synthase gene from varieties of Pennisetum species

Genet Mol Res. 2015 Mar 31;14(1):2799-808. doi: 10.4238/2015.March.31.10.

Abstract

Sucrose phosphate synthase (SPS) is an enzyme used by higher plants for sucrose synthesis. In this study, three primer sets were designed on the basis of known SPS sequences from maize (GenBank: NM_001112224.1) and sugarcane (GenBank: JN584485.1), and five novel SPS genes were identified by RT-PCR from the genomes of Pennisetum spp (the hybrid P. americanum x P. purpureum, P. purpureum Schum., P. purpureum Schum. cv. Red, P. purpureum Schum. cv. Taiwan, and P. purpureum Schum. cv. Mott). The cloned sequences showed 99.9% identity and 80-88% similarity to the SPS sequences of other plants. The SPS gene of hybrid Pennisetum had one nucleotide and four amino acid polymorphisms compared to the other four germplasms, and cluster analysis was performed to assess genetic diversity in this species. Additional characterization of the SPS gene product can potentially allow Pennisetum to be exploited as a biofuel source.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular
  • Cluster Analysis
  • DNA, Complementary / chemistry
  • DNA, Complementary / genetics
  • Genetic Variation*
  • Genome, Plant / genetics
  • Glucosyltransferases / genetics*
  • Molecular Sequence Data
  • Pennisetum / classification
  • Pennisetum / enzymology
  • Pennisetum / genetics*
  • Phylogeny
  • Plant Proteins / genetics*
  • Polymorphism, Single Nucleotide
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Sequence Homology, Nucleic Acid
  • Species Specificity

Substances

  • DNA, Complementary
  • Plant Proteins
  • Glucosyltransferases
  • sucrose-phosphate synthase