HSA-based phosphorescent probe for two-photon in vitro visualization

Pavel S Chelushkin; Natalia V Nukolova; Alexei S Melnikov; Pavel Yu Serdobintsev; Pavel A Melnikov; Dmitry V Krupenya; Igor O Koshevoy; Sergey V Burov; Sergey P Tunik

doi:10.1016/j.jinorgbio.2015.03.014

HSA-based phosphorescent probe for two-photon in vitro visualization

J Inorg Biochem. 2015 Aug:149:108-11. doi: 10.1016/j.jinorgbio.2015.03.014. Epub 2015 Apr 1.

Authors

Pavel S Chelushkin¹, Natalia V Nukolova², Alexei S Melnikov³, Pavel Yu Serdobintsev³, Pavel A Melnikov⁴, Dmitry V Krupenya⁵, Igor O Koshevoy⁶, Sergey V Burov⁷, Sergey P Tunik⁸

Affiliations

¹ Institute of Chemistry, Saint Petersburg State University, Universitetskii Pr. 26, Saint Petersburg 198504, Russia; Institute of Macromolecular Compounds, Russian Academy of Sciences, Bolshoi Pr. V.O., 31, Saint Petersburg 199004, Russia. Electronic address: chelushkin@gmail.com.
² Serbsky State Scientific Center for Social and Forensic Psychiatry, Kropotkinsky Per, 25, Moscow 119991, Russia; Pirogov Russian National Research Medical University, Ostrovitianov St. 1, Moscow 117997, Russia.
³ Department of Physics, Saint Petersburg State University, Ulianovskaya St. 3, Saint Petersburg 198504, Russia; Research Institute of Nanobiotechnologies, Saint Petersburg State Polytechnical University, 29, Polytechnicheskaya St., Saint Petersburg 195251, Russia.
⁴ Pirogov Russian National Research Medical University, Ostrovitianov St. 1, Moscow 117997, Russia.
⁵ Institute of Chemistry, Saint Petersburg State University, Universitetskii Pr. 26, Saint Petersburg 198504, Russia.
⁶ Department of Chemistry, University of Eastern Finland, Joensuu 80101, Finland.
⁷ Institute of Chemistry, Saint Petersburg State University, Universitetskii Pr. 26, Saint Petersburg 198504, Russia; Institute of Macromolecular Compounds, Russian Academy of Sciences, Bolshoi Pr. V.O., 31, Saint Petersburg 199004, Russia.
⁸ Institute of Chemistry, Saint Petersburg State University, Universitetskii Pr. 26, Saint Petersburg 198504, Russia. Electronic address: stunik@inbox.ru.

PMID: 25864999
DOI: 10.1016/j.jinorgbio.2015.03.014

Abstract

Two-photon microscopy reveals several advantages over conventional one since it provides higher spatial resolution as well as deeper penetration into the sample under study. The development of suitable two-photon probes is one of the most challenging tasks in this area. Here we present phosphorescent non-covalent adduct of human serum albumin and Au-Ag alkynyl-diphosphine complex, [Au14Ag4(C2Ph)12(PPh2C6H4PPh2)6][PF6]4, which exhibits high cross section of two-photon-induced luminescence (δTPE) within large near-infrared excitation wavelength region (700-800 nm) with maximum δTPE about 38 GM at 740 nm. This feature makes it a promising probe for multiphoton bioimaging as demonstrated by successful visualization of glioma C6 cells and various tissues by two-photon confocal microscopy both in planar and z-stacking modes. Additionally, the broad excitation region enables optimization of the signal-to-background auto-fluorescence ratio via variation of excitation wavelength.

Keywords: Human serum albumin; Imaging agents; Luminescence; Non-covalent interactions; Non-linear optics.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Albumins / chemistry*
Cell Line, Tumor
Gold / chemistry
Humans
Luminescent Agents / chemical synthesis*
Luminescent Agents / chemistry
Microscopy, Fluorescence, Multiphoton / methods
Organogold Compounds / chemical synthesis*
Organogold Compounds / chemistry
Silver / chemistry

Substances

Albumins
Luminescent Agents
Organogold Compounds
Silver
Gold