This paper presents current views on the persistence of immunity following vaccination against hepatitis B. Very high effectiveness of hepatitis B vaccination has been reported in a number of studies worldwide. Standard vaccination with approved schedule induces protective antibody titers in healthy newborns, children, adolescents and adults in more than 96% and 90% of cases, respectively. A number of studies have also confirmed the occurrence of anamnestic response to a booster injection of HB vaccine even after 20 years following primary immunization. From the numerous studies transpires that cellular response following hepatitis B vaccination persists longer compared to humoral response. Irrespective of gradual decline and loss of anti-HBs antibodies, adequately performed primary immunization in healthy persons ensures long-term protection against acute and chronic stages of hepatitis B. In fact, T and B lymphocytes, whose responsiveness prevails the presence of anti-HBs antibodies in serum, are true markers of immunity. A special attention should be given to persons with secondary immunodeficiencies or immunosuppressed patients whose immunization against hepatitis B raises difficulties.