[Detection of flavivirus in mosquitoes (Diptera: Culicidae) from Easter Island-Chile]

Rev Chilena Infectol. 2015 Feb;32(1):113-6. doi: 10.4067/S0716-10182015000200021.
[Article in Spanish]

Abstract

Flaviviruses are arthropod-borne viruses, mainly by mosquitoes of the genera Aedes and Culex (Culicidae) that are detected in tropical and subtropical areas. Main flaviviruses of public health importance are: dengue, West Nile virus, yellow fever, among others. In continental Chile, flaviviruses has not been detected. However, there are indigenous cases of dengue detected in Easter Island since 2002, as the presence of its vector Aedes aegypti. The aim of this study was: To determine diversity of flavivirus mosquitoes present in Easter Island. Thirty pools of mosquitoes collected in Hanga Roa were analyzed; a RT-PCR nested flavivirus was performed. Thirteen positive samples were detected and the amplification products were sequenced, identifying two specific flavivirus Insect, the Cell fusing agent virus and other related viruses Kamiti River. This is the first study in Chile showed the presence of flavivirus in vectors in Easter Island.

Publication types

  • English Abstract

MeSH terms

  • Animals
  • Chile
  • Culicidae / virology*
  • Flavivirus / genetics
  • Flavivirus / isolation & purification*
  • Humans
  • Insect Vectors / virology*
  • Phylogeny
  • Polynesia
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Analysis, DNA