A novel ECL biosensor for β-lactamase detection: Using RU(II) linked-ampicillin complex as the recognition element

Guo-Feng Gui; Ying Zhuo; Ya-Qin Chai; Yun Xiang; Ruo Yuan

doi:10.1016/j.bios.2015.03.023

A novel ECL biosensor for β-lactamase detection: Using RU(II) linked-ampicillin complex as the recognition element

Biosens Bioelectron. 2015 Aug 15:70:221-5. doi: 10.1016/j.bios.2015.03.023. Epub 2015 Mar 10.

Authors

Guo-Feng Gui¹, Ying Zhuo², Ya-Qin Chai³, Yun Xiang², Ruo Yuan⁴

Affiliations

¹ Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China; College of Chemical Engineering, Guizhou University of Engineering Science, Guizhou 551700, China.
² Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.
³ Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China. Electronic address: yqchai@swu.edu.cn.
⁴ Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China. Electronic address: yuanruo@swu.edu.cn.

PMID: 25841118
DOI: 10.1016/j.bios.2015.03.023

Abstract

In this work, Ru(phen)2(cpaphen)(2+) linked-ampicillin (Ru-Amp), as the novel specific recognition element, was proposed to construct a simple and sensitive electrogenerated chemiluminescence (ECL) biosensor for the determination of β-lactamase. Here, Ru-Amp complex acted not only as a novel specific recognition element for β-lactamase but also as the ECL Luminescent reagent. Through electrostatic adsorption and the intermolecular π-π interactions, a large amount of Ru-Amp was immobilized to gold nanoparticles (TA@AuNPs) prepared by thiophenemalonic acid (TA) to obtain Ru-Amp/TA@AuNPs nanocomposites. The nanocomposites, which can produce very stable films exhibiting excellent ECL behaviors, were self-assembled on the CNTs-Nf modified glassy carbon electrode surface. The presence of the target β-lactamase resulted in autonomous hydrolysis reaction of Amp, achievement of the efficient ECL emission and highly sensitive detection of β-lactamase. The biosensor for β-lactamase detection was developed with excellent sensitivity of a concentration variation from 50 pg mL(-1) to 100 ng mL(-1) with a low detection limit of 37 pg mL(-1). An ECL assay offers the proposed method opportunities for designing new Ru-based ECL luminophores for biosensing applications.

Keywords: ECL biosensor; Ru-Amp; TA@AuNPs; β-Lactamase.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Ampicillin / chemistry*
Biosensing Techniques / instrumentation*
Conductometry / instrumentation*
Electrodes
Equipment Design
Equipment Failure Analysis
Luminescent Measurements / instrumentation*
Reproducibility of Results
Ruthenium / chemistry*
Sensitivity and Specificity
beta-Lactamases / analysis*
beta-Lactamases / chemistry

Substances

Ampicillin
Ruthenium
beta-Lactamases