An allelic exchange vector was constructed to replace gfp by mCherry in bacteria previously tagged with mini-Tn5 derivatives. The method was successfully applied to a gfp-labeled Yersinia pseudotuberculosis strain and the re-engineered bacterium was used to study the colonization of Steinernema nematodes hosting their Xenorhabdus symbiont using dual-color confocal microscopy.
Keywords: Allelic exchange; Confocal microscopy; Fluorescent protein; GFP; Gram-negative bacteria; Suicide plasmid; mCherry.
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