(1)H NMR spectroscopy and multiple linear regression analysis were used to determine diesel fuel oxidation stability. Oxidation stability is one of the most important properties which affect diesel fuel characteristics and quality. It has been demonstrated that integrated peak intensities of certain functional groups in (1)H NMR spectra correlated well with the oxidation stability. This approach is fast, nondestructive and requires small amounts of samples. It has a potential to be used as an alternative to existing standard methods.