Abstract
The aim of the work reported herein was to investigate the effect of various low molecular weight chitosans (LMWCs) on the stability of insulin using USP HPLC methods. Insulin was found to be stable in a polyelectrolyte complex (PEC) consisting of insulin and LMWC in the presence of a Tris-buffer at pH 6.5. In the presence of LMWC, the stability of insulin increased with decreasing molecular weight of LMWC; 13 kDa LMWC was the most efficient molecular weight for enhancing the physical and chemical stability of insulin. Solubilization of insulin-LMWC polyelectrolyte complex (I-LMWC PEC) in a reverse micelle (RM) system, administered to diabetic rats, results in an oral delivery system for insulin with acceptable bioactivity.
MeSH terms
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Administration, Oral
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Animals
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Biological Availability
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Biotransformation
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Chitin / analogs & derivatives*
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Chitin / chemistry
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Chitosan
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Diabetes Mellitus, Experimental / blood
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Diabetes Mellitus, Experimental / drug therapy*
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Diabetes Mellitus, Experimental / metabolism
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Drug Delivery Systems*
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Drug Liberation
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Drug Stability
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Drug Storage
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Electrolytes / chemistry*
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Excipients / chemistry*
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Hyperglycemia / prevention & control
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Hypoglycemic Agents / administration & dosage*
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Hypoglycemic Agents / blood
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Hypoglycemic Agents / pharmacokinetics
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Hypoglycemic Agents / therapeutic use
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Insulin / administration & dosage*
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Insulin / analogs & derivatives
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Insulin / analysis
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Insulin / blood
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Insulin / pharmacokinetics
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Insulin / therapeutic use
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Male
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Micelles
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Molecular Weight
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Oligosaccharides
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Particle Size
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Random Allocation
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Rats, Sprague-Dawley
Substances
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Electrolytes
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Excipients
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Hypoglycemic Agents
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Insulin
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Micelles
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Oligosaccharides
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insulin, desamido (A21)-
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oligochitosan
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Chitin
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Chitosan