DNA polymerase-catalyzed incorporation of nucleotides modified with a G-quadruplex-derived DNAzyme

Chem Commun (Camb). 2015 Apr 30;51(34):7379-81. doi: 10.1039/c5cc01387a.

Abstract

We show that nucleotides which are modified with a G-quadruplex-derived DNAzyme are substrates for DNA polymerases. Based on this finding we developed a naked-eye detection system that allows the detection of single nucleotide variations in DNA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Benzothiazoles / chemistry
  • Biocatalysis
  • Biosensing Techniques
  • DNA, Catalytic / chemistry*
  • DNA, Catalytic / metabolism*
  • DNA-Directed DNA Polymerase / metabolism*
  • G-Quadruplexes*
  • Models, Molecular
  • Nucleotides / metabolism*
  • Oxidation-Reduction
  • Polymorphism, Single Nucleotide
  • Sulfonic Acids / chemistry

Substances

  • Benzothiazoles
  • DNA, Catalytic
  • Nucleotides
  • Sulfonic Acids
  • 2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid
  • DNA-Directed DNA Polymerase