Tutorial review on validation of liquid chromatography-mass spectrometry methods: part II

Anneli Kruve; Riin Rebane; Karin Kipper; Maarja-Liisa Oldekop; Hanno Evard; Koit Herodes; Pekka Ravio; Ivo Leito

doi:10.1016/j.aca.2015.02.016

Tutorial review on validation of liquid chromatography-mass spectrometry methods: part II

Anal Chim Acta. 2015 Apr 22:870:8-28. doi: 10.1016/j.aca.2015.02.016. Epub 2015 Feb 13.

Authors

Anneli Kruve¹, Riin Rebane¹, Karin Kipper¹, Maarja-Liisa Oldekop¹, Hanno Evard¹, Koit Herodes¹, Pekka Ravio², Ivo Leito³

Affiliations

¹ University of Tartu, Institute of Chemistry, Ravila 14a, 50411 Tartu, Estonia.
² Finnish Customs Laboratory, Tekniikantie 13, PL 53, FI-02151 Espoo, Finland.
³ University of Tartu, Institute of Chemistry, Ravila 14a, 50411 Tartu, Estonia. Electronic address: ivo.leito@ut.ee.

PMID: 25819784
DOI: 10.1016/j.aca.2015.02.016

Abstract

This is the part II of a tutorial review intending to give an overview of the state of the art of method validation in liquid chromatography mass spectrometry (LC-MS) and discuss specific issues that arise with MS (and MS-MS) detection in LC (as opposed to the "conventional" detectors). The Part II starts with briefly introducing the main quantitation methods and then addresses the performance related to quantification: linearity of signal, sensitivity, precision, trueness, accuracy, stability and measurement uncertainty. The last section is devoted to practical considerations in validation. With every performance characteristic its essence and terminology are addressed, the current status of treating it is reviewed and recommendations are given, how to handle it, specifically in the case of LC-MS methods.

Keywords: Electrospray; Ionization suppression; Limit of detection; Liquid chromatography–mass spectrometry; Robustness; Validation.

Publication types

Review