Bufadienolides and polyhydroxycholestane derivatives from Bufo bufo gargarizans

J Asian Nat Prod Res. 2015;17(4):364-76. doi: 10.1080/10286020.2014.995174. Epub 2015 Mar 30.

Abstract

A new polyhydroxycholestane sulfate ester, 3α,12β,25,26-tetrahydroxy-7-oxo-5β-cholestane 26-O-sulfate (1), was isolated from dried skin of Bufo bufo gargarizans Cantor and its structure was elucidated on the basis of extensive 1D and 2D NMR as well as HR-ESI-MS analysis. A comparison of steroidal metabolite profiles, based on HPLC and LC-MS analyses, indicates that the chemical compositions of the various parts of toads, such as venom, skin and stratum corneum, are significantly different.

Keywords: Bufo bufo gargarizans; bufadienolide; polyhydroxycholestane sulfate; toad skin; toad venom.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bufanolides / chemistry
  • Bufanolides / isolation & purification*
  • Bufo bufo*
  • China
  • Cholestanols / chemistry
  • Cholestanols / isolation & purification*
  • Chromatography, High Pressure Liquid
  • Molecular Structure
  • Nuclear Magnetic Resonance, Biomolecular
  • Skin / chemistry
  • Venoms

Substances

  • 3alpha,12beta,25,26-tetrahydroxy-7-oxo-5beta-cholestane 26-O-sulfate
  • Bufanolides
  • Cholestanols
  • Venoms