Biochemical analysis and hypoglycemic activity of a polysaccharide isolated from the fruit of Lycium barbarum L

Hua-Li Tang; Chen Chen; Shao-Kang Wang; Gui-Ju Sun

doi:10.1016/j.ijbiomac.2015.03.026

Biochemical analysis and hypoglycemic activity of a polysaccharide isolated from the fruit of Lycium barbarum L

Int J Biol Macromol. 2015:77:235-42. doi: 10.1016/j.ijbiomac.2015.03.026. Epub 2015 Mar 26.

Authors

Hua-Li Tang¹, Chen Chen², Shao-Kang Wang², Gui-Ju Sun³

Affiliations

¹ Key Laboratory of Environmental Medicine and Engineering of Ministry of Education, and Department of Nutrition and Food Hygiene, School of Public Health, Southeast University, Nanjing 210009, PR China; School of Life Science and Engineering, Chongqing Three Gorges University, Chongqing 404000, PR China.
² Key Laboratory of Environmental Medicine and Engineering of Ministry of Education, and Department of Nutrition and Food Hygiene, School of Public Health, Southeast University, Nanjing 210009, PR China.
³ Key Laboratory of Environmental Medicine and Engineering of Ministry of Education, and Department of Nutrition and Food Hygiene, School of Public Health, Southeast University, Nanjing 210009, PR China. Electronic address: 40649716@qq.com.

PMID: 25819220
DOI: 10.1016/j.ijbiomac.2015.03.026

Abstract

Purification, characterization and hypoglycemic activities of polysaccharide from Lycium barbarum L. were investigated in this study. A water soluble polysaccharide (LBP) was obtained with ultrafiltration membranes separation, which was further purified by chromatography of DEAE cellulose column and Sephadex G-150 to get LBP3a and LBP3b. The high performance permeation chromatography (HPGPC) analysis showed that the average molecular weight (Mw) of LBP3b was 4.92kDa. Monosaccharide composition analysis revealed that the LBP3b was comprised of mannose, rhamnose, glucose, galactose and xylose with a molar ratio of 5.52:5.11:28.06:1.00:1.70. The preliminary structure features of LBP3b were investigated by UV, FT-IR, NMR and SEM. In vitro cell experiments showed that LBP3b had significantly inhibited the absorption of glucose in a dose-dependent manner. The study showed that LBP3b had potential use as an anti-diabetic agent.

Keywords: Characterization; Hypoglycemic activity; Lycium barbarum L.; Polysaccharide.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Caco-2 Cells
Fruit / chemistry*
Glucose / metabolism
Humans
Hypoglycemic Agents / chemistry*
Hypoglycemic Agents / isolation & purification
Hypoglycemic Agents / pharmacology*
Lycium / chemistry*
Molecular Weight
Monosaccharides / analysis
Polysaccharides / chemistry*
Polysaccharides / isolation & purification
Polysaccharides / pharmacology*
Ultrafiltration

Substances

Hypoglycemic Agents
Monosaccharides
Polysaccharides
Glucose