Utilization of a reporter system based on the blue pigment indigoidine biosynthetic gene bpsA for detection of promoter activity and deletion of genes in Streptomyces

Renata Knirschova; Renata Novakova; Erik Mingyar; Carmen Bekeova; Dagmar Homerova; Jan Kormanec

doi:10.1016/j.mimet.2015.03.017

Utilization of a reporter system based on the blue pigment indigoidine biosynthetic gene bpsA for detection of promoter activity and deletion of genes in Streptomyces

J Microbiol Methods. 2015 Jun:113:1-3. doi: 10.1016/j.mimet.2015.03.017. Epub 2015 Mar 20.

Authors

Renata Knirschova¹, Renata Novakova¹, Erik Mingyar¹, Carmen Bekeova¹, Dagmar Homerova¹, Jan Kormanec²

Affiliations

¹ Institute of Molecular Biology, Slovak Academy of Sciences, 845 51 Bratislava, Slovak Republic.
² Institute of Molecular Biology, Slovak Academy of Sciences, 845 51 Bratislava, Slovak Republic. Electronic address: jan.kormanec@savba.sk.

PMID: 25801098
DOI: 10.1016/j.mimet.2015.03.017

Abstract

The integrative promoter-probe plasmid pBPSA1 was constructed using a promoterless Streptomyces aureofaciens CCM3239 bpsA gene encoding a non-ribosomal peptide synthase for the biosynthesis of a blue pigment, indigoidine. bpsA was also used to prepare pAMR4 plasmid for the deletion of genes in Streptomyces with facile identification of double crossover recombination.

Keywords: Actinorhodin; Gene disruption; Indigoidine; Promoter-probe vector; Reporter gene; Streptomyces.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Genes, Bacterial*
Genes, Reporter*
Peptide Synthases / genetics
Piperidones / metabolism*
Plasmids
Promoter Regions, Genetic*
Recombination, Genetic
Sequence Deletion*
Streptomyces aureofaciens / enzymology
Streptomyces aureofaciens / genetics*

Substances

Piperidones
indigoidine
Peptide Synthases
non-ribosomal peptide synthase