Comparative proteomic analysis in Miscanthus sinensis exposed to antimony stress

Liang Xue; Huadong Ren; Sheng Li; Ming Gao; Shengqing Shi; Ermei Chang; Yuan Wei; Xiaohua Yao; Zeping Jiang; Jianfeng Liu

doi:10.1016/j.envpol.2015.03.004

Comparative proteomic analysis in Miscanthus sinensis exposed to antimony stress

Environ Pollut. 2015 Jun:201:150-60. doi: 10.1016/j.envpol.2015.03.004. Epub 2015 Mar 21.

Authors

Liang Xue¹, Huadong Ren², Sheng Li², Ming Gao¹, Shengqing Shi³, Ermei Chang³, Yuan Wei³, Xiaohua Yao², Zeping Jiang³, Jianfeng Liu⁴

Affiliations

¹ Research Institute of Subtropical Forestry, Chinese Academy Forestry, Fuyang, Zhejiang 311400, China; State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China.
² Research Institute of Subtropical Forestry, Chinese Academy Forestry, Fuyang, Zhejiang 311400, China.
³ State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China.
⁴ State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China. Electronic address: liujf@caf.ac.cn.

PMID: 25800729
DOI: 10.1016/j.envpol.2015.03.004

Abstract

To explore the molecular basis of Sb tolerance mechanism in plant, a comparative proteomic analysis of both roots and leaves in Miscanthus sinensis has been conducted in combination with physiological and biochemical analyses. M. sinensis seedlings were exposed to different doses of Sb, and both roots and leaves were collected after 3 days of treatment. Two-dimensional gel electrophoresis (2-DE) and image analyses found that 29 protein spots showed 1.5-fold change in abundance in leaves and 19 spots in roots, of which 31 were identified by MALDI-TOF-MS and MALDI-TOF-TOF-MS. Proteins involved in antioxidant defense and stress response generally increased their expression all over the Sb treatments. In addition, proteins relative to transcription, signal transduction, energy metabolism and cell division and cell structure showed a variable expression pattern over Sb concentrations. Overall these findings provide new insights into the probable survival mechanisms by which M. sinensis could be adapting to Sb phytotoxicity.

Keywords: Antimony; Miscanthus sinensis; Proteomic; Stress.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antimony / pharmacology*
Electrophoresis, Gel, Two-Dimensional
Environmental Exposure / analysis
Plant Leaves / drug effects*
Plant Proteins / metabolism
Plant Roots / drug effects*
Poaceae / drug effects*
Proteomics / methods*
Seedlings / drug effects
Signal Transduction / genetics
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Stress, Physiological*

Substances

Plant Proteins
Antimony