Functional analysis of the engineered cardiac tissue grown on recombinant spidroin fiber meshes

PLoS One. 2015 Mar 23;10(3):e0121155. doi: 10.1371/journal.pone.0121155. eCollection 2015.

Abstract

In the present study, we examined the ability of the recombinant spidroin to serve as a substrate for the cardiac tissue engineering. For this purpose, isolated neonatal rat cardiomyocytes were seeded on the electrospun spidroin fiber matrices and cultured to form the confluent cardiac monolayers. Besides the adhesion assay and immunostaining analysis, we tested the ability of the cultured cardiomyocytes to form a functional cardiac syncytium by studying excitation propagation in the cultured tissue with the aid of optical mapping. It was demonstrated that recombinant spidroin fiber meshes are directly suitable for the adherence and growth of the cardiomyocytes without additional coating with the attachment factors, such as fibronectin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Adhesion
  • Cell Proliferation
  • Cells, Cultured
  • Fibroins / genetics
  • Fibroins / metabolism*
  • Myocytes, Cardiac / cytology*
  • Myocytes, Cardiac / metabolism
  • Rats
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism
  • Tissue Engineering / methods*
  • Tissue Scaffolds

Substances

  • Recombinant Proteins
  • Fibroins

Grants and funding

The work was in part funded by the Russian Government Program “5 TOP-100” and research grant from MIPT. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.